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a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus <t>IX83)</t> and b) a second microscope using different hardware (Leica DM6000).
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inverted microscope frame  (Olympus)
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a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus <t>IX83)</t> and b) a second microscope using different hardware (Leica DM6000).
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inverted microscope frame  (Evident Corporation)
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a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus <t>IX83)</t> and b) a second microscope using different hardware (Leica DM6000).
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a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus <t>IX83)</t> and b) a second microscope using different hardware (Leica DM6000).
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a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus IX83) and b) a second microscope using different hardware (Leica DM6000).

Journal: Genetics

Article Title: Automated cell lineage reconstruction using label-free 4D microscopy

doi: 10.1093/genetics/iyae135

Figure Lengend Snippet: a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus IX83) and b) a second microscope using different hardware (Leica DM6000).

Article Snippet: Imaging was performed using either DIC alone for N2 or both DIC and fluorescence for JIM113 using an Olympus IX83 inverted frame equipped with a UPLSAPO60xs2 objective, a Visitech iSIM multipoint confocal scanner, ASI MX2000XYZ stage, and Hamamatsu Orca Fusion camera.

Techniques: Microscopy